Diagnostic Testing
Bacterial V3V4 16S rRNA Amplicon Sequencing
Bacterial 16S V3-V4 amplicon sequencing for research profiling of marker-gene reads; it is not a gut-health or infection diagnosis.
About this service
The named service sequences the V3-V4 region of the bacterial 16S rRNA gene to characterize bacterial community marker data in an agreed sample. Results are affected by primer choice, copy number, amplification, reference databases and bioinformatics; closely related bacteria may not resolve to species, and read proportions are not direct cell counts.
Service highlights
- Targets the bacterial 16S rRNA V3-V4 marker region.
- Research profiling of bacterial community sequence data.
- Resolution and relative abundance depend on method and references.
Who may consider it
- Microbiome researchers.
- Laboratories studying bacterial communities.
What to expect
Confirm current project availability and quote. Agree the sample matrix and whether the provider accepts a specimen, extracted DNA, amplicons/library or raw reads; confirm extraction, PCR/library preparation, sequencing, analysis, controls and deliverables. No sample SOP or turnaround is provided.
Scope and limitations
16S amplicon sequencing profiles marker-gene reads, not every microorganism or a complete microbial genome. It does not reliably quantify organisms or establish viability, infection, disease causation or treatment choice.
Frequently asked questions
What does V3-V4 16S sequencing do?
It sequences selected bacterial 16S marker regions for research characterization of a community.
Does it identify and count every species?
No. Taxonomic resolution varies and read proportions are not direct cell counts; primer and database biases apply.
Can it diagnose gut disease or infection?
No clinical diagnostic use is established. Confirm input requirements, availability and quote.